![]() ![]() These temperature differences may have an effect on gamete/embryo development. In addition, temperatures between the front and back of each shelf and among the fronts and backs of all shelves were significantly different. Temperatures between incubators and among shelves were significantly different. With the use of wireless sensors, we were able to evaluate temperature in two similar front-loading incubators. The study objective was to investigate temperature variation within a front-loading incubator. ![]() When we combined the values for the fronts and backs of the three shelves for the two incubators, temperatures were still significantly different ( P < .001 36.80 ☌ ± 0.14 ☌ for the top shelves, 36.71 ☌ ± 0.19 ☌ for the middle shelves, and 36.78 ☌ ± 0.07 ☌ for the bottom shelves). ![]() Furthermore, the same was true for the backs of these shelves ( P < .001 Table 1). When we evaluated the front of the shelves of both incubators separately, we found them to be significantly different ( P < .001 Table 1). When the three shelves were evaluated as separate units, temperatures between the fronts and backs of shelves were significantly different (Table 1 Fig. Inside temperatures between the fronts and backs of incubators (all shelves included) were significantly different (36.85 ☌ ± 0.15 ☌ in the front of the incubators versus 36.68 ☌ ± 0.08 ☌ in the back P < .001). We pooled the data from both incubators for all of the following analyses. ![]() With the use of wireless temperature probes, we evaluated temperatures between the front and the back of incubators, between and among incubator shelves, and between incubators. The aim of this study is to investigate temperature differentials inside front-loading incubators typically used in ART laboratories. The reason for improved embryo development on the middle shelf is unknown, but may be caused by temperature variations. Higdon and associates reported that human embryos cultured on middle shelves of front-loading incubators had better odds of generating a pregnancy compared with embryos placed on the top or bottom shelves. However, within an incubator, internal temperature differentials between and among shelves may exist. Generally, among ART laboratories, temperature is recorded once daily at one position inside an incubator. Embryo culture below 37 ☌ is not as detrimental however, embryo development is slowed. Rat embryos cultured 2 ☌ above normal body temperature (normal body temperature of rats is 38.0 ☌) demonstrated altered embryonic development and organogenesis. In rat models, Kimmel and associates demonstrated that embryos were adversely influenced by elevated temperature and that an increase in culture temperature could be lethal. Abramczuk and Lopata found the highest human in vitro embryo cleavage progression rate and pregnancy rate were obtained at 36.9☌. Eng and associates reported in vivo maturation of pig oocytes occurs better at 39 ☌ than at 37 ☌. Previous reports confirm that variations among temperature can alter embryo development. Because temperature has a direct impact on embryo homeostasis, it is important to monitor and control temperatures in the ART laboratory. Scientists have conducted numerous studies to investigate the affect temperature has on embryo development and pregnancy rates. To have a successful Assisted Reproductive Technology (ART) program, there are many variables that laboratory personnel must take into consideration. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |